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KO/KD
Validated
Validated
Description
Research Area
Cancer Biology, Cell Biology, Epigenetics & Chromatin, Metabolism Research, Signal Transduction
Images & Validation
−
Item 1 of 4
| Tested Applications | ELISA, FC, ICC, IF, KO/KD Validated, WB |
|---|---|
| Dilution Range | Western blot, 0.1-0.5 μg/ml Immunocytochemistry/Immunofluorescence, 5 μg/ml Immunoprecipitation, 0.5-2 μg/ml Flow Cytometry (Fixed), 1-3μg/1x10^6 cells ELISA, 0.1-0.5 μg/ml |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | E. coli-derived human NF-kB p65 recombinant protein (Position: F99-S551). |
| Target | Transcription factor p65 |
| Molecular Weight | 70 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−NF Kappa B; NF κB; NFKB 3; NFKB p65; NFKB3; NF-κB; NF-κB3; p65; RELA; Transcription factor p65
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Quick Database Links
Gene Symbol
Transcription factor p65
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Available Sizes
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Free Secondary Antibody (20 ul)0/0
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