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Description
Research Area
Cancer Biology
Images & Validation
−
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:20,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000 |
| Reactivity | Bovine, Goat, Guinea pig, Hamster, Human, Monkey, Mouse, Porcine, Rat, Sheep |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | beta-Actin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 350-375 of Human beta Actin. |
| Target | ACTB |
| Purity | Beta Actin Antibody Peroxidase Conjugated antibody is directed against human beta Actin protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates and some yeast. Broad reactivity makes this antibody an excellent loading control. |
| Conjugation | HRP |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.0 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-beta Actin Antibody HRP conjugation, peroxidase conjugated rabbit anti-beta Actin Antibody, Actin Antibody, Loading Control Antibody, beta actin, ß actin, anti-beta actin antibody
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Quick Database Links
Gene Symbol
ACTB
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Available Sizes
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