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Biotin-11-dCTP

SKU: orb64039

Description

Biotin-11-dCTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal sub-strate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of cytidine. Recommended Biotin-11-dCTP/dCTP ratio for PCR and Nick Translation: 50% Biotin-11-dCTP/50% dCTP.

Images & Validation

Application Notes
FISH; SNP-analysis; Nick-translation; TUNEL.
Spectroscopic Propertie: λmax 294 nm, ε 9.3 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

Key Properties

CAS Number136632-30-9
MWTheoretical MW: 859.67 g/mol (free acid); Detected MW: 859.18 g/mol (free acid)
Purity≥ 95% (HPLC)
FormulaC28H44N7O16P3S
SMILESN(CC#Cc1c(nc(=O)n([C@H]2C[C@@H]([C@H](O2)COP(=O)(OP(=O)(OP(=O)(O)O)O)O)O)c1)N)C(=O)CCCCCNC(=O)CCCC[C@H]1[C@H]2NC(=O)N[C@H]2CS1

Customer Validated Data

The data below is submitted by researchers worldwide. We provide this transparency to help you decide if this product works for your specific application or species, even if we haven't tested it ourselves.
Success by Application
Reactivity Distribution

Latest Experiments

5 Results
Dilution
Sample
Summary
Labeling
Mouse
-
Erythroleukaemia G1E‑ER4 cells
The Biotin‑11‑dCTP is used in the...
Biotin fill‑in of chromatin ends
Coral
1 mM
Chromatin from coral cells
The Biotin‑11‑dCTP is used in the...
Labelling
Mouse
-
Mouse muscle stem cells
Biotin‑11‑dCTP is used in the Micro‑C...
Labelling
Human
-
K562 cells
Biotin‑11‑dCTP is used in the Micro‑C...
Micro‑C library preparation
Human
1 mM
Granta519 cells, DND41 cells.
This reagent is used in the...

Storage & Handling

Storagestore at -20 °C
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Biotin-X-5-Propargylamino-dCTP, γ-[N-(Biotin-6-amino-hexanoyl)]-5-propargylamino-2'-deoxycytidine-5'-triphosphate, Triethylammonium salt

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Quality Guarantee

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Key Properties

No computed properties available.

Protocol Information

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5 x 200 μl
$ 570.00
DispatchUsually dispatched within 5-10 working days
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