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Description
Research Area
Cell Biology
Images & Validation
−
Item 1 of 4
| Tested Applications | FC, IF, IHC-Fr, IHC-P, WB |
|---|---|
| Dilution Range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg /Test |
| Reactivity | Human, Rat |
| Predicted Reactivity | Bovine, Canine, Equine, Mouse, Porcine |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from rat Caspase-8 subunit p10 (411-482/482aa) |
| Target | CASP8 |
| Molecular Weight | 57 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ALPS2B; CAP4; Casp-8; FLICE; MACH; MCH5; CD20; CK-20; CK20; K20; KRT21; CASP8_HUMAN; CASP8; Apoptotic cysteine protease; Apoptotic protease Mch-5; FADD-homologous ICE/ced-3-like protease; FADD-like ICE (FLICE); ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog (MACH); 3.4.22.61; CASP8_MOUSE; CASP8_RAT; caspase 8; caspase 8, apoptosis-related cysteine protease; caspase 8, apoptosis-related cysteine peptidase
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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Quick Database Links
Gene Symbol
CASP8
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
IF
Immunofluorescence
Filter by Applications
Filter by Species
Vanesa Stojanovska, Anzari Atik, Ilias Nitsos, Béatrice Skiöld, Samantha K. Barton, Valerie A. Zahra, Karyn Rodgers, Stuart B. Hooper, Graeme R. Polglase, Robert Galinsky Effects of Intrauterine Inflammation on Cortical Gray Matter of Near-Term Lambs Frontiers in Pediatrics, 6, 145 (2018)
Applications
IHC
Reactivity
Sheep
Barton, Samantha K. et al. Differential short-term regional effects of early high dose erythropoietin on white matter in preterm lambs after mechanical ventilation J Physiol, 594, 1437-1449 (2016)
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Free Secondary Antibody (20 ul)0/0
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