Collagen IV antibody

SKU: orb2276392

This product can be custom made

Description

Collagen IV antibody validated for immunohistochemistry on 76 different Normal Tissues

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

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Item 1 of 3

Tested Applications	IHC
Dilution Range	1:100-1:200
Reactivity	Human
Application Notes	Positive Control: Colon: An at least moderate collagen IV immunostaining of basement membranes and a strong staining of vessels and the tunica muscularis mucosa should be seen. Negative Control: Colon: Collagen IV immunostaining must be absent in epithelial cells. Cellular Localization: Cytoplasmic and extracellular Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit according to the manufacturer’s directions.

Key Properties

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Host	Rabbit
Clonality	Monoclonal
Isotype	IgG
Clone No.	MSVA-704R
Immunogen	Purified human placental extract
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Arresten; BSVD; COL4A1; COL4A1 NC1 domain; COL4A2; COL4A3; COL4A4; COL4A5; collagen alpha-1(IV) chain; Collagen IV Alpha 1 Polypeptide; Collagen IV Alpha 2 Polypeptide; Collagen Type IV Alpha 2; Collagen Type IV Alpha 3; Collagen Type IV Alpha 4; Collagen Type IV Alpha 5; RATOR

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Quick Database Links

UniProt

P29400, P08572, P02462, P53420

UniProt Details

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No UniProt data available

Protocol Information

IHC

Immunohistochemistry

View Protocol

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Applications:	IF, IHC-Fr, IHC-P, WB
Predicted Reactivity:	Bovine, Canine, Gallus, Sheep
Reactivity:	Human, Mouse, Rabbit, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	ELISA, ICC, IHC, WB
Reactivity:	Human, Mouse, Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	DOT, ELISA, IHC, IP, WB
Reactivity:	Bovine, Human
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	DOT, ELISA, IHC, IP, WB
Reactivity:	Bovine, Human
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Applications:	DOT, ELISA, IHC, IP, WB
Reactivity:	Bovine, Human
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Unconjugated

Collagen IV antibody

Description

Research Area

Images & Validation

Key Properties

Storage & Handling

Alternative Names

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Quality Guarantee

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UniProt Details

Protocol Information

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