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Description
Research Area
Epigenetics & Chromatin
Images & Validation
−
Item 1 of 3
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:10,000-1:50,000, IHC: 5µg/mL, WB: 1:500-1:2,000 |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | This affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of CTCF protein. |
| Target | CTCF |
| Purity | This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with endogenous CTCF protein. A BLAST analysis was used to suggest reactivity with CTCF from human, mouse, horse, bovine, panda, rabbit, Danio, and chicken based on a 100% homology with the immunizing sequence. Cross-reactivity with CTCF from other sources has not been determined. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.0 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-CTCF Antibody, Transcriptional repressor CTCF, 11-zinc finger protein, CCCTC-binding factor, CTCFL paralog
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_006556.1 |
|---|
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)