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Description
Research Area
Epigenetics, Non-Animal
Images & Validation
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| Application Notes |
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Key Properties
−| Source | E.coli |
|---|---|
| Biological Origin | Corynephage beta |
| Biological Activity | ①After DT3C 10μg/ml formed complexes with different concentrations of Anti-CCR8 antibodies, cytotoxicity experiments were conducted on CHOK1/Human CCR8 cells. The ED50 is 0.3708-0.5608 μg/ml. ②After DT3C 10μg/ml formed complexes with different concentrations of Anti-CCR8 antibodies, cytotoxicity experiments were conducted on CT26/Human CCR8 cells. The ED50 is 0.4661-0.8614 μg/ml. |
| Tag | N-terminal 6xHis-tagged |
| Expression Region | 33-417aa(P00588)+linker(GGGGSGGGGSGGGGS)+291-497aa(P19909) |
| Protein Length | Heterodimer |
| Endotoxins | Less than 1.0 EU/μg as determined by LAL method. |
| MW | 69.4 kDa |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Protein Sequence | GADDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYDDDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKELGLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEINFETRGKRGQDAMYEYMAQACAGNRVRRSVGSSLSCINLDWDVIRDKTKTKIESLKEHGPIKNKMSESPNKTVSEEKAKQYLEEFHQTALEHPELSELKTVTGTNPVFAGANYAAWAVNVAQVIDSETADNLEKTTAALSILPGIGSVMGIADGAVHHNTEEIVAQSIALSSLMVAQAIPLVGELVDIGFAAYNFVESIINLFQVVHNSYNRPAYSPGHKGGGGSGGGGSGGGGSIDEILAALPKTDTYKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDDATKTFTVTEKPEVIDASELTPAVTTYKLVINGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDDATKTFTVTEKPEVIDASELTPAVTTYKLVINGKTLKGETTTKAVDAETAEKAFKQYANDNGVDGVWTYDDATKTFTVTE |
Storage & Handling
−| Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
|---|---|
| Form/Appearance | Lyophilized powder |
| Buffer/Preservatives | Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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Recombinant Corynephage beta DT3C protein, partial [orb1785188]
Greater than 85% as determined by SDS-PAGE.
65.0 kDa
E.coli
1 mg, 100 μg, 20 μg
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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