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Genomics & Molecular Biology
Explore CRISPR
A comprehensive guide to high-precision genetic engineering, essential active Cas enzymes, and critical gRNA methodologies driving modern biotechnology.
At Biorbyt, we are at the forefront of the biotech revolution, pioneering breakthroughs in genetic engineering through CRISPR technology. Whether you're a seasoned researcher in a prestigious university or a forward-thinking company seeking to leverage the power of CRISPR, we have the products and expertise to accelerate your projects and drive innovation.
1. What is CRISPR?
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) technology has fundamentally revolutionized the field of genetic engineering. Inspired by the natural adaptive defense mechanisms of bacteria against invading bacteriophage viruses, CRISPR allows scientists to precisely edit DNA with unprecedented accuracy, efficiency, and scale.
Mechanism of Action
CRISPR works by precisely targeting and cutting specific sequences of DNA with the help of a specialized endonuclease enzyme, like Cas9. This programmed cut can be clinically utilized to either cleanly disrupt a target gene's function (knockout) or introduce highly desired changes (knock-in) by providing a specific DNA template for homology-directed repair. The cell's natural repair mechanisms then permanently incorporate the desired changes, enabling precise gene editing.
2. Why Choose CRISPR from Biorbyt?
We offer a comprehensive range of highly validated CRISPR products, including active recombinant CRISPR enzymes, streamlined CRISPR kits, and custom-designed gRNAs tailored specifically to your unique research needs.
3. Active CRISPR Enzymes
Choose from a wide selection of active CRISPR enzymes, globally renowned for their precision and efficiency in genome editing. Our enzymes are produced recombinantly in E. coli with full-length, sequence-verified constructs.
| Cat. Code | Enzyme Name | Bacterial Source | Purification Tag |
|---|---|---|---|
| orb1742858 | AapCas12b, Active | Alicyclobacillus acidiphilus | Tag Free |
| orb1742857 | AsCas12a, Active | Acidaminococcus sp. | His |
| orb1742856 | Cas12 (LbCpf1), Active | Lachnospiraceae | GST |
| orb1742855 | EiCsm6, Active | Enterococcus italicus | His |
| orb1742854 | LbuCas13a, Active | Leptotrichia buccalis | Tag Free |
| orb1821057 | LbuCas13a, Active (Glycerol-free) | Leptotrichia buccalis | Tag Free |
| orb1742853 | LwCas13a, Active | Leptotrichia wadei | His |
| orb1742852 | PsmCas13b, Active | Prevotella sp. | His |
| orb1742851 | SpCas9 (D10A)-NLS | Streptococcus pyogenes | His |
| orb1742850 | SpCas9 (D10A, H840A)-NLS | Streptococcus pyogenes | His |
| orb1742849 | SpCas9 (H840A)-NLS | Streptococcus pyogenes | His |
| orb1742848 | SpCas9, Active | Streptococcus pyogenes | His |
| orb1742847 | SpCas9-NLS, Active | Streptococcus pyogenes | His |
| orb1742846 | SpCas9-NLS (Glycerol-free) | Streptococcus pyogenes | His |
| orb1742845 | TccCas13a, Active | Thermoclostridium caenicola | His |
| orb1742844 | TtCsm6, Active | Thermus thermophilus | His |
CRISPR Core Reagents
We provide high-quality CRISPR reagents and highly validated buffers to functionally support your gene editing experiments, ensuring reliable and reproducible sequence alteration.
4. Kits & Custom gRNAs
Streamline your workflow with our ready-to-use CRISPR kits, designed to massively simplify experimental setup and optimization. Whether you're performing clinical genome editing, knockout screening, or functional genomics studies, our kits provide everything you need for success.
Cas12 (AapCas12b) Kit
Cas12 (LbCpf1, GST) Kit
Cas12 (LbCpf1, His) Kit
Cas13 [LwCas13a (LwC2c2)] Kit
Cas13 (LbuCas13a) Kit
Custom Guide RNAs (gRNAs) Service
Tailor your CRISPR experiments to your specific chromosomal targets with our custom gRNA synthesis service. Our team of bioinformatic experts can design and synthesize highly stable gRNAs optimized specifically for your target genes, ensuring precise, off-target-free genome editing.
5. Applications of CRISPR
Explore the massively diverse applications of CRISPR technology across the biosciences:
Therapeutic Development
Harness the profound therapeutic potential of CRISPR for directly treating genetic disorders, highly evasive cancers, and viral infectious diseases. Researchers are developing precise, targeted gene therapies and personalized medicine approaches to revolutionize healthcare.
Functional Genomics & Research
Unravel the extreme complexity of gene function and regulatory networks utilizing CRISPR-based high-throughput screening and functional profiling techniques. From creating precise in vivo disease models to validating potential drug targets, CRISPR opens entirely new avenues for biomedical research.
Agricultural Biotechnology
Enhance critical crop traits, improve overall yields, and develop highly disease-resistant varieties utilizing CRISPR-mediated genome editing. This technology is revolutionizing modern agriculture by creating more resilient and sustainable crops to address global food security.