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Caspase 3 Antibody

SKU: orb125009
This product can be custom made

Description

Goat polyclonal antibody to Caspase 3

Research Area

Cell Biology

Images & Validation

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Tested ApplicationsELISA, IF, WB
Dilution RangeELISA: 1:32000, WB: 0.5-1 μg/ml, IHC-P: 10 μg/ml
ReactivityHuman, Mouse, Rat
Predicted ReactivityCanine
Application Notes
WB: Approx 32kDa band observed in lysates of cell line MOLT4 and in Mouse Liver lysates (calculated MW of 31.6kDa according to Human NP_004337.2 and 31.4kDa according to Mouse NP_033940.1, NP_001271338.1). An additional band of 65kDa was also consistently ob. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 cells. Recommended concentration: 10µg/ml. This antibody has been successfully used in ICC on Rat: Hóngyi Zhào et al. (2018) PMID: 29853981.

Key Properties

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ClonalityPolyclonal
TargetCaspase 3
Protein SequenceRDVSKEDHSKRS
Molecular Weight31.6
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

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StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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anti Apopain antibody, anti CASP 3 antibody, anti CASP-3 antibody, anti CASP3 antibody, anti CC3 antibody, anti CPP 32 antibody, anti CPP-32 antibody, anti CPP32 antibody, anti CPP32B antibody, anti ICE3 antibody, anti LICE antibody, anti PARP cleavage protease antibody, anti Procaspase3 antibody, anti Protein Yama antibody, anti Yama antibody, anti Yama protein antibody, anti Caspase-3 antibody, anti Caspase3 antibody, anti caspase3 antibody, anti caspase 3 antibody

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Quick Database Links

Gene Symbol
Caspase 3
NCBI Gene ID
836

NCBI Gene Details

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No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
Find More Protocols

Available Sizes

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100 μg
$ 560.00
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Free Secondary Antibody (20 ul)0/0

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