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Item 1 of 4
| Tested Applications | ELISA, FC, IF, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:32000, WB: 0.5-2 μg/ml, IHC-P: 3.75 μg/ml |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Canine, Mouse, Porcine, Rat |
| Application Notes |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Target | IGF1 |
| Protein Sequence | RSVRAQRHTD |
| Molecular Weight | 17.8; 15.2; 21.8; 17.0 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti IGF1 antibody, anti insulin-like growth factor 1 (somatomedin C) antibody, anti IGF1A antibody, anti IGFI antibody, anti OTTHUMP00000195084 antibody, anti insulin-like growth factor 1 antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Wang, Yan et al. Rumen-Protected Glucose Stimulates the Insulin-Like Growth Factor System and mTOR/AKT Pathway in the Endometrium of Early Postpartum Dairy Cows Animals (Basel), 10, E357 (2020)
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