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LITAF Antibody

SKU: orb19631

Description

Goat polyclonal antibody to LITAF

Research Area

Neuroscience

Images & Validation

Tested ApplicationsELISA, IHC
Dilution RangeELISA: 1:8000, WB: 0.1 μg/ml, IHC-P: 6.5 μg/ml
ReactivityHuman
Predicted ReactivityMouse, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Preliminary experiments gave an approx 25kDa band in Human Lymph Node lysates and in lysates of cell lines HeLa and HepG2 after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 17.1kDa according to NP_004853.2. The 25kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

ClonalityPolyclonal
TargetLITAF
Protein SequencePDGKGMNPPSYYTQ
Molecular Weight17.1; 15.8
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

LITAF; lipopolysaccharide-induced TNF factor; CMT1C; FLJ38636; MGC116698; MGC116700; MGC116701; MGC125274; MGC125275; MGC125276; PIG7; SIMPLE; TP53I7; LPS-induced TNF-alpha factor; small integral membrane protein of lysosome/late endosome; tumor protein

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NCBI Gene Details

No NCBI Gene data available

Protocol Information

IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 560.00
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Free Secondary Antibody (20 ul)0/0

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