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Human CLEC4E protein

SKU: orb391486

Description

Recombinant human CLEC4E protein is produced by our Mammalian expression system and the target gene encoding Arg41-Leu219 is expressed with a 6His tag at the C-terminus.

Research Area

Immunology & Inflammation

Images & Validation

Tested ApplicationsELISA, MS, SDS-PAGE, WB
Application Notes
Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

SourceHuman cells
TargetCLEC4E
ReactivityHuman
EndotoxinsLess than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
PurityGreater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Protein SequenceRCVVTFRIFQ TCDEKKFQLP ENFTELSCYN YGSGSVKNCC PLNWEYFQSS CYFFSTDTIS WALSLKNCSA MGAHLVVINS QEEQEFLSYK KPKMREFFIG LSDQVVEGQW QWVDGTPLTK SLSFWDVGEP NNIATLEDCA TMRDSSNPRQ NWNDVTCFLN YFRICEMVGI NPLNKGKSLV DHHHHHH

Storage & Handling

StorageLyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/AppearanceLyophilized
Buffer/Preservatives0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CLECSF9; MINCLE; C-type lectin domain family 4 member E; C-type lectin superfamily member 9; Macrophage-inducible C-type lectin

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

Protocol Information

Protein Handling and Storage Guide
Protein Handling Guide
View Guide
WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
View Protocol

Available Sizes

Select a size below

10 μg
$ 300.00
50 μg
$ 680.00
500 μg
$ 3,230.00
DispatchUsually dispatched within 5-10 working days
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