Human Corticotropin Releasing Hormone (CRH) ELISA Kit

SKU: orb2667901

Description

The Human Corticotropin Releasing Hormone (CRH) ELISA Kit is designed to quantify Corticotropin Releasing Hormone (CRH) in human serum, plasma and other biological fluids. The kit has a detection range of 0.32-20 ng/mL and a sensitivity of 0.13 ng/mL.

Research Area

Metabolism Research; Pharmacology & Drug Discovery

Images & Validation

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Tested Applications

ELISA

Application Notes

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human CRH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CRH in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

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Reactivity	Human
Sample Types	serum, plasma and other biological fluids
Assay Type	Competitive
Assay Time	2h
Range	0.32-20 ng/mL
Sensitivity	0.13 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CRF; Corticotropin Releasing Factor; Corticoliberin

Quality Guarantee

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Quick Database Links

Gene Symbol

CRH

UniProt

P06850

UniProt Details

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No UniProt data available

Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

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