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Description
Research Area
Signal Transduction
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Source | Sf9 Insect Cells |
|---|---|
| Biological Activity | ~125-175 units/mg. One unit of MEK1 activity transfers 1 nmol of phosphate to ERK1/2 peptide per minute at 30°C in a reaction containing 100µM ATP. Recombinant active MEK1 also phosphorylates ERK1, ERK2, and GSK-3?. Kinase activity may vary depending on the substrate and reaction conditions. The optimal concentration should be determined for each specific application. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
Storage & Handling
−| Storage | Stability: Store at 4°C if entire vial will be used within 1-2 weeks. Store frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles |
|---|---|
| Form/Appearance | Sterile Filtered clear solution. |
| Buffer/Preservatives | MEK1 is supplied at a concentration of in 40mM Tris, pH-8, 0.15M NaCl, 0.27M sucrose, 1mM DTT, 0.2mM PMSF, 1mM benzamidine, 0.1mM sodium vanadate and 0.03% Brij-35. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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