Human Pepsin (PP) ELISA Kit

SKU: orb782228

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Description

The Human Pepsin (PP) ELISA Kit is designed to quantify Pepsin (PP) in human serum, plasma and other biological fluids. The kit has a detection range of 3.13-200 ng/mL and a sensitivity of 0.93 ng/mL.

Research Area

Cancer Biology, Gastroenterology & Hepatology, Protein Biochemistry

Images & Validation

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Tested Applications

ELISA

Application Notes

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PP in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma and other biological fluids
Assay Type	Competitive
Assay Time	2h
Range	3.13-200 ng/mL
Sensitivity	0.93 ng/mL
Concentration	200 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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PP ELISA Kit, Pepsin ELISA Kit

Quality Guarantee

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

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