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Description
Research Area
Immunology & Inflammation
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | ED50 is approximately 2 ng/ml as determined by the dose-dependent induction of IL-6 in primary human foreskin fibroblasts.Specific Activity of 5 x 105 IU/mg. |
| Target | IL17A |
| Tag | Tag Free |
| MW | 15.26 kD |
| Purity | Greater than 95% as determined by reducing SDS-PAGE.Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. |
| Protein Sequence | Ile20-Ala155 |
Storage & Handling
−| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Lyophilized from a 0.2 um filtered solution of 20mM PB, pH 7.4. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Interleukin-17A, IL-17, IL-17A, Cytotoxic T-Lymphocyte-Associated Antigen 8, CTLA-8, IL17A, CTLA8, IL17
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The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 30.7 kDa after removal of the signal peptide. The apparent molecular mass of TEM1 -His is approximately 35-55 kDa due to glycosylation.
Mammalian
50 μg, 100 μg, 10 μg Recombinant Human IL-17RA Protein [orb2994535]
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SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.
Predicted: 60.6 KDa. Observed: 90-120 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human IL-17A Protein [orb2994915]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (Regularly tested)
Predicted: 15.9 KDa. Observed: 15-22 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human IL-17A Protein [orb2995247]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 15.26 KDa. Observed: 16 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human IL17RC Protein [orb2992710]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Predicted: 75 KDa. Observed: 80-140 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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