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Description
Research Area
Immunology & Inflammation
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Source | Human Cells |
|---|---|
| Target | IL5 |
| Tag | C-6 His tag |
| MW | 14.2 kD |
| Purity | Greater than 95% as determined by reducing SDS-PAGE.Endotoxin level less than 0.1 ng/ug (1 IEU/ug) as determined by LAL test. |
| Protein Sequence | Ile20-Ser134 |
Storage & Handling
−| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Lyophilized from a 0.2 um filtered solution of PBS,pH7.4. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Interleukin-5, IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF, IL5
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Human IL5 Protein, His Tag [orb689428]
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The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 13.94 kDa after removal of the signal peptide.The apparent molecular mass of IL5-His is approximately 15-25 kDa due to glycosylation.
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100 μg, 50 μg, 10 μg Recombinant Human IL-5RA Protein [orb2994170]
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 36.7 KDa. Observed: 45-60 KDa, reducing conditions
1 mg, 10 μg, 50 μg, 500 μgRecombinant Human IL-5RA Protein [orb2994171]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 62.5 KDa. Observed: 70-85 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Mouse IL-5RA Protein [orb2994722]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 37.6 KDa. Observed: 48 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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