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Description
Research Area
Immunology & Inflammation
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | Maintains pluripotency of mouse and human embryonic stem cells. The LIF (GST-tagged) from AdipoGen has the same activity as cleaved LIF. |
| Target | LIF |
| Tag | GST tag |
| Purity | >95% (SDS-PAGE). Endotoxin level is less than 0.05EU/ μg purified protein (LAL test; Lonza). |
| Protein Sequence | Human LIF (aa 23-202) is fused at the N-terminus to a tag. |
Storage & Handling
−| Storage | Stable for at least 6 months after receipt when stored at -20°C. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Contains PBS. Reconstitute with 100 μl sterile water. |
| Concentration | 0.1mg/ml after reconstitution |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Leukemia Inhibitory Factor, Differentiation-stimulating Factor, D Factor, Melanoma-derived LPL Inhibitor
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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