Mouse Ceruloplasmin (CP) ELISA Kit

SKU: orb2667892

Description

The Mouse Ceruloplasmin (CP) ELISA Kit is designed to quantify Ceruloplasmin (CP) in mouse serum, plasma and other biological fluids. The kit has a detection range of 1.57-100 µg/mL and a sensitivity of 0.59 µg/mL.

Research Area

Infectious Disease & Virology, Metabolism Research

Images & Validation

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Application Notes

standard: 100 µg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Mouse
Sample Types	Serum, plasma and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	1.57-100 µg/mL
Sensitivity	0.59 µg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CP2; CER; Caeruloplasmin; Ferroxidase; Oxygen Oxidoreductase

Quality Guarantee

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Quick Database Links

UniProt

Q61147

UniProt Details

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No UniProt data available

Protocol Information

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Sensitivity:	1.88 ng/mL

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Dynamic Range:	0.781-50 ng/mL
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