Mouse Thyrotropin Releasing Hormone (TRH) ELISA Kit

SKU: orb776506

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Description

The Mouse Thyrotropin Releasing Hormone (TRH) ELISA Kit is designed to quantify Thyrotropin Releasing Hormone (TRH) in mouse serum, plasma and other biological fluids. The kit has a detection range of 7.82-500 pg/mL and a sensitivity of 2.69 pg/mL.

Research Area

Metabolism Research

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse TRH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TRH in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

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Reactivity	Mouse
Sample Types	serum, plasma and other biological fluids
Assay Type	Competitive
Assay Time	2h
Range	7.82-500 pg/mL
Sensitivity	2.69 pg/mL
Concentration	500 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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TRF ELISA Kit, Thyrotropin-Releasing Factor ELISA Kit, Thyroliberin ELISA Kit, Protirelin ELISA Kit, Pro-tirelin ELISA Kit, Prothyroliberin ELISA Kit, TSH-releasing factor ELISA Kit

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Quick Database Links

Gene Symbol

TRH

UniProt

Q62361

UniProt Details

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No UniProt data available

Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols

Reactivity:	Mouse
Dynamic Range:	7.82-500pg/mL
Sensitivity:	4.69 pg/mL

Reactivity:	Mouse
Dynamic Range:	7.81-500pg/mL
Sensitivity:	2.89pg/mL

Reactivity:	Mouse
Dynamic Range:	2.5 pg/mL-160 pg/mL
Sensitivity:	0.625 pg/mL

Reactivity:	Mouse
Dynamic Range:	7.81-500 pg/mL
Sensitivity:	2.77 pg/mL

Reactivity:	Mouse
Dynamic Range:	7.812-500 pg/mL
Sensitivity:	2.77 pg/mL