NLRP3-IN-84

NLRP3-IN-84 (Compound 32) is an inhibitor of the NLRP3 inflammasome. It interferes with the oligomerization of NLRP3 by inhibiting the activity of NLRP3ATPase (IC50= 158.4 nM). NLRP3-IN-84 also inhibits Caspase-1 (IC50= 27.7 nM), the release of IL-1β in PBMC (IC50= 19.5 nM) and mPBMC (IC50= 24.2 nM), and ASC speck formation (IC50= 131 nM). It shows no inhibitory activity against the NLRC4 and AIM2 inflammasomes. NLRP3-IN-84 exhibits significant in vivo anti-inflammatory effects in a mouse acute peritonitis model, making it useful for studying NLRP3-related inflammatory diseases.