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NSE gamma antibody

SKU: orb2276527

Description

NSE gamma antibody validated for immunohistochemistry on 76 different Normal Tissues

Research Area

Metabolism Research

Images & Validation

Tested ApplicationsIHC
Dilution Range1:100-1:200
ReactivityHuman
Application Notes
Positive Control: In the colon, axons and ganglion cells in lamina propria and muscular wall must show at least a moderate NSE staining, while epithelial and lymphatic cells remain negative. Negative Control: In the colon, epithelial and lymphatic cells do not show NSE staining. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit according to the manufacturer’s directions.

Key Properties

HostMouse
ClonalityMonoclonal
IsotypeIgG1, kappa
Clone No.MSVA-451M
ImmunogenA synthetic peptide of human NSE gamma (around aa416-433) (exact sequence is proprietary)
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

ENO2; ENOG; Enolase 2 gamma neuronal; Enolase2; Gamma-enolase; Neural enolase; Neuron specific gamma enolase; Neuron-specific enolase; NSE ; -phospho-D-glycerate hydrolyase

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UniProt Details

No UniProt data available

Protocol Information

IHC
Immunohistochemistry
View Protocol

Available Sizes

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0.1 ml
$ 370.00
0.5 ml
$ 740.00
1 ml
$ 1,100.00
Free Secondary Antibody (20 ul)0/0

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