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Description
Research Area
Signal Transduction
Images & Validation
−
Item 1 of 4
| Tested Applications | FC, IP, WB |
|---|---|
| Dilution Range | WB=1:500-2000, Flow-Cyt=2µg/Test, IP=1:50-200 |
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human Occludin (151-250/522aa) |
| Target | OCLN |
| Molecular Weight | 65 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Ocl; BLCPMG; PPP1R115; PTORCH1; OCLN_CANLF; OCLN; OCLN_HUMAN; OCLN_MOUSE; OCLN_RAT; occludin; tight junction protein occludin TM4 minus; phosphatase 1, regulatory subunit 115
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Quick Database Links
Gene Symbol
OCLN
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IP
Immunoprecipitation
Filter by Applications
Filter by Species
Tsai-Kun Wu, Paik-Seong Lim, Jong-Shiaw Jin, Ming-Ying Wu, Chang-Hsu Chen Impaired Gut Epithelial Tight Junction Expression in Hemodialysis Patients Complicated with Intradialytic Hypotension Biomed Res Int ., 2018, 1 (2018)
Applications
IHC
Reactivity
Human
Elham Zahedi 1 2, Fatemeh Mokhtari Naseri 3, Elham Zamani 4, Marjan Nikbakhtzadeh 5, Tayebeh Rastegar 6, Ashkan Sanaeirad 7, Seyed Shahabeddin Sadr Ginger Extract Improves Cognitive Dysfunction via Modulation of Gut Microbiota-Derived Short-Chain Fatty Acids in D-Galactose/Ovariectomy-Induced Alzheimer-Like Disease Mol Neurobiol, (2024)
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Free Secondary Antibody (20 ul)0/0
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