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Description
Research Area
Signal Transduction
Images & Validation
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Item 1 of 4
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:3,000 - 1:12,000, IHC: 1:200 - 1:1,000, WB: 1:200 - 1:2,000 |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-Polo-like Kinase pT210 Antibody was produced by repeated immunizations with a synthetic phospho peptide corresponding to an internal region near aa 200-225 of Human Polo-like kinase 1 (Plk1) protein. |
| Target | PLK1 |
| Purity | Anti-Polo-like Kinase pT210 Antibody is directed against human phosphorylated Plk1 protein. This antibody was affinity purified. This antibody is specific for phosphorylated human Plk-1 protein at the pT210 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with Plk-1 homologues from human, chimpanzee, pig, chicken, mouse, rat, Xenopus, dog, mosquito, zebra fish, starfish, sea urchin and fruit fly. Cross reactivity with Plk-1 protein homologues from C.elgans and honeybee may also occur as sequence homology varies by one amino acid residue in this sequence. Reactivity with Plk-1 protein from other sources is not known. Minimal reactivity is expected with the non-phosphorylated form of the protein. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.0 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-PLK1 pT210 antibody, Serine/threonine-protein kinase PLK1, rabbit anti-polio like kinase 1 antibody, Serine/threonine protein kinase 13 antibody, PLK-1, PLK 1, STPK13
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)