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EPCAM Antibody (Biotin)

SKU: orb750871

Description

Mouse monoclonal antibody against EpCAM conjugated to Biotin

Research Area

Immunology & Inflammation

Images & Validation

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Tested ApplicationsFC, IF, IHC
Dilution RangeFlow Cytometry (5ul per test per one million cells or 5ul per 100ul of whole blood); Immunofluorescence (2-4ug/ml);,Immunohistochemistry (Formalin-fixed) (2-4ug/ml at RT);,(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
ReactivityHuman
Application Notes
Positive Control: HT29 cells or breast tumor

Key Properties

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HostMouse
ClonalityMonoclonal
IsotypeIgG1
Clone No.VU-1D9
ImmunogenSmall cell lung carcinoma cells
TargetEPCAM
Source/Expression SystemBioreactor
Molecular Weight40-43kDa
PurificationProtein G
ConjugationBiotin

Storage & Handling

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StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Buffer/PreservativesAntibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
ConcentrationPurified Ab conjugated to Biotin
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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Anti-Adenocarcinoma-associated Antigen antibody, anti-Cell Surface Glycoprotein Trop-1 antibody, anti-EGP2 antibody, anti-EGP314 antibody, anti-EGP40 antibody, anti-Epithelial Cell Adhesion Molecule antibody, anti-Epithelial Glycoprotein 314 antibody, anti-ESA antibody, anti-KSA antibody, anti-TACD1 antibody, anti-TROP1 antibody, anti-Tumor-associated Calcium Signal Transducer 1 (TACSTD1) antibody, anti-ECS-1 antibody, anti-Epidermal Surface Antigen 1 antibody, anti-ESA1 antibody, anti-FLOT2 antibody, anti-Flotillin-2 antibody, anti-Membrane Component antibody, anti-Chromosome 17 antibody, anti-Surface Marker-1 (M17S1) antibody, anti-REG-1 antibody, anti-Reggie-1 antibody, anti-Reggie-2 antibody

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UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

Protocol Information

IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol

Available Sizes

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0.5 ml
$ 680.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 5-10 working days
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