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Description
Research Area
Cell Biology
Images & Validation
−
Item 1 of 6
| Tested Applications | FC, ICC, IF, IHC, WB |
|---|---|
| Dilution Range | WB (1:1000), IHC (1:100), ICC/IF (1:100), FCM (1:100); optimal dilutions for assays should be determined by the user. |
| Reactivity | Bovine, Drosophila, Frog, Gallus, Human, Mouse, Rat, Sheep |
| Application Notes |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Immunogen | A 35 residue synthetic peptide, corresponding to Rat Erk1 MAP kinase with the CGG spacer group added and the peptide coupled to KLH. |
| Target | Erk1/2 |
| Molecular Weight | 42kDa (ERK2). |
| Purification | Peptide Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS pH 7.4, 50% glycerol, 0.09% sodium azide. Storage buffer changes when conjugated. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ERK1, ERK2, ERT1, ERT2, MAP kinase 1, MAP kinase 2, MAPK1, MAPK2, MAPK3, p38, p40, p41, p41mapk, p42 MAPK, p44 ERK1, p44 MAPK, PRKM1, PRKM2, PRKM3
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_059043.1 |
|---|
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
Filter by Applications
Filter by Species
Su-Ji Min, Hye-Won Hyun, Tae-Cheon Kang Leptomycin B attenuates neuronal death via PKA- and PP2B-mediated ERK1/2 activation in the rat hippocampus following status epilepticus Brain Research, 1670, 14 (2017)
Applications
WB
Reactivity
Rat
Available Sizes
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