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Human Afamin (AFM) ELISA Kit

SKU: orb776872
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Description

The Human Afamin (AFM) ELISA Kit uses a sandwich enzyme immunoassay to quantify Afamin (AFM) in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The kit has a detection range of 3.13-200 ng/mL and a sensitivity of 1.54 ng/mL.

Research Area

Cancer Biology, Gastroenterology & Hepatology, Infectious Disease & Virology

Images & Validation

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Tested ApplicationsELISA
Application Notes
standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AFM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AFM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AFM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AFM in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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ReactivityHuman
Sample TypesSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay TypeSandwich
Assay Time3.5h
Range3.13-200 ng/mL
Sensitivity1.54 ng/mL
Concentration200 ng/mL

Storage & Handling

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StorageRefer to the Storage Guidelines in the Manual
Expiration DatePlease enquire.
DisclaimerFor research use only

Alternative Names

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ALB2 ELISA Kit, ALBA ELISA Kit, ALF ELISA Kit, A-ALB ELISA Kit, Alpha-Albumin ELISA Kit

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Quick Database Links

UniProt
P43652

UniProt Details

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No UniProt data available

Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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Available Sizes

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48 T
$ 330.00
96 T
$ 470.00
DispatchUsually dispatched within 5-10 working days
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