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Human Collagen Type III Alpha 1 (COL3a1) ELISA Kit

SKU: orb776763
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Description

The Human Collagen Type III Alpha 1 (COL3a1) ELISA Kit uses a sandwich enzyme immunoassay to quantify Collagen Type III Alpha 1 (COL3a1) in human serum, plasma, tissue homogenates and other biological fluids. The kit has a detection range of 0.63-40 ng/mL and a sensitivity of 0.245 ng/mL.

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

Tested ApplicationsELISA
Application Notes
standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COL3a1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COL3a1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COL3a1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COL3a1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

ReactivityHuman
Sample TypesSerum, plasma, tissue homogenates and other biological fluids
Assay TypeSandwich
Assay Time3.5h
Range0.63-40 ng/mL
Sensitivity0.245 ng/mL
Concentration40 ng/mL

Storage & Handling

StorageRefer to the Storage Guidelines in the Manual
Expiration DatePlease enquire.
DisclaimerFor research use only

Alternative Names

COL3-A1 ELISA Kit, COL3A1 ELISA Kit, EDS4A ELISA Kit, Collagen Type III Alpha 1 ELISA Kit, Ehlers-Danlos Syndrome Type IV Autosomal Dominant ELISA Kit, Collagen Alpha-1(III)chain ELISA Kit

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

UniProt Details

No UniProt data available

Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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48 T
$ 330.00
96 T
$ 470.00
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