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Human N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) EasyStep ELISA Kit

SKU: orb1950025
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Description

The Human N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) EasyStep ELISA Kit uses a sandwich enzyme immunoassay to quantify N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) EasyStep in human serum, plasma. The kit has a detection range of 0.94-60 ng/mL and a sensitivity of 0.94 ng/mL.

Research Area

Neuroscience; Cardiovascular Research

Images & Validation

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Human N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) EasyStep ELISA Kit
Application Notes
standard: 60 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NT-ProBNP, and the Human NT-ProBNP standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human NT-ProBNP. After TMB substrate solution is added, only those wells that contain Human NT-ProBNP and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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ReactivityHuman
Sample TypesSerum, plasma
Assay TypeSandwich
Assay Time1.5h
Range0.94-60 ng/mL
Sensitivity0.94 ng/mL
Concentration60 ng/mL

Storage & Handling

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StorageRefer to the Storage Guidelines in the Manual
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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NT-Pro-BNP; N-BNP; ANFB_HUMAN; BNP(1-32); BNP(5-29); BNP-32; Gamma-brain natriuretic peptide; nppb
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Protocol Information

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48 T
$ 330.00
96 T
$ 470.00
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