Human Plasminogen Activator Inhibitor 1 (PAI1) ELISA Kit

SKU: orb2668077

Description

The Human Plasminogen Activator Inhibitor 1 (PAI1) ELISA Kit is designed to quantify Plasminogen Activator Inhibitor 1 (PAI1) in human plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The kit has a detection range of 1.56-100 ng/mL and a sensitivity of 0.53 ng/mL.

Research Area

Pharmacology & Drug Discovery

Images & Validation

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Application Notes

standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PAI1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PAI1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PAI1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PAI1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	1.56-100 ng/mL
Sensitivity	0.53 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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SERPINE1; PLANH1; Serpin Peptidase Inhibitor Clade E Member 1; Nexin,Plasminogen Activator Inhibitor Type 1 Serpin E1; Endothelial plasminogen activator inhibitor

Quality Guarantee

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Quick Database Links

UniProt

P05121

UniProt Details

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No UniProt data available

Protocol Information

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