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Human Regenerating Islet Derived Protein 3 Alpha (REG3a) ELISA Kit

SKU: orb777284
Free Kit Promotion

Description

The Human Regenerating Islet Derived Protein 3 Alpha (REG3a) ELISA Kit uses a sandwich enzyme immunoassay to quantify Regenerating Islet Derived Protein 3 Alpha (REG3a) in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The kit has a detection range of 31.25-2000 pg/mL and a sensitivity of 12.5 pg/mL.

Research Area

Immunology & Inflammation

Images & Validation

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Tested ApplicationsELISA
Application Notes
standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human REG3a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human REG3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human REG3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human REG3a in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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ReactivityHuman
Sample Typesserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay TypeSandwich
Assay Time3.5h
Range31.25-2000 pg/mL
Sensitivity12.5 pg/mL
Concentration2000 pg/mL

Storage & Handling

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StorageRefer to the Storage Guidelines in the Manual
Expiration DatePlease enquire.
DisclaimerFor research use only

Alternative Names

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HIP ELISA Kit, INGAP ELISA Kit, PAP-H ELISA Kit, PAP1 ELISA Kit, PBCGF ELISA Kit, REG-III ELISA Kit, REG3 ELISA Kit, Pancreatitis-Associated Protein ELISA Kit, Hepatointestinal pancreatic protein ELISA Kit, Human proislet peptide ELISA Kit, Pancreatitis-associated protein 1 ELISA Kit

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

UniProt Details

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No UniProt data available

Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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48 T
$ 330.00
96 T
$ 470.00
DispatchUsually dispatched within 5-10 working days
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