IkB-a (Phospho-Ser32/Ser36) Rabbit Polyclonal Antibody

SKU: orb14868

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Description

This is a rabbit polyclonal antibody targeting IkB-a. It is unconjugated and suitable for use in IF, IHC, WB applications. The antibody is reactive with human, mouse samples. Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

Research Area

Signal Transduction

Images & Validation

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Tested Applications	IF, IHC, WB
Dilution Range	WB: 1：500 - 1：1000; IHC: 1：100 - 1：200; IF: 1：100 - 1：500
Reactivity	Human, Mouse

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Immunogen	Peptide-KLH. Peptide sequence around phosphorylation site of serine 32/36 (H-D-S(p)-G-L-D-S(p)-M-K) derived from Human IkB-a
Target	IkB-a
Purification	Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Concentration	1.0mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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I-kappa-B-alpha; IKBA; NF-kappaB inhibitor alpha; NFKBI; NFKBIA

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