IGFBP3 Antibody (Biotin)

SKU: orb1272742

This product can be custom made

Description

Research Area

Cell Biology

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, WB
Reactivity	Human
Application Notes	ELISA:Sandwich:To detect hIGF-BP3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human IGF-BP3 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP3. Western Blot:To detect hIGF-BP3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Immunogen	Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIGF-BP3. Human IGF-BP3 specific antibody was purified by affinity chromatography and then biotinylated.
Target	IGFBP3
Conjugation	Biotin

Storage & Handling

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Storage	IGF-BP3 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Form/Appearance	Lyophilized
Concentration	batch dependent
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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IBP3, BP-53, IBP3, Insulin-like growth factor-binding protein 3IBP-3

Quality Guarantee

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Quick Database Links

Gene Symbol

IGFBP3

UniProt

P17936

RefSeq:P17936

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

RefSeq	P17936

Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Find More Protocols

Applications:	IF, IHC-Fr, IHC-P
Predicted Reactivity:	Human, Mouse
Reactivity:	Rat
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Biotin

Applications:	WB
Predicted Reactivity:	Mouse
Reactivity:	Human, Rat
Species/Host:	Mouse
Clonality:	Monoclonal
Conjugation:	Biotin

Applications:	WB
Predicted Reactivity:	Mouse, Rat
Reactivity:	Human
Species/Host:	Rabbit
Clonality:	Polyclonal
Conjugation:	Biotin

IGFBP3 Antibody (Biotin)

Description

Research Area

Images & Validation

Key Properties

Storage & Handling

Alternative Names

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