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Il4 Antibody (Biotin)

SKU: orb1671458

Description

Il4 Antibody (Biotin)

Research Area

Immunology & Inflammation

Images & Validation

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Tested ApplicationsELISA, WB
ReactivityMouse
Application Notes
Sandwich ELISA: To detect Murine IL-4 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Biorbyt’s Polyclonal Anti-Murine IL-4 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Murine IL-4. Western Blot To detect Murine IL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Murine IL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Key Properties

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Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
ImmunogenProduced from sera of rabbits pre-immunized with highly pure recombinant Murine IL-4. Anti-Murine IL-4 specific antibody was purified by affinity chromatography and then biotinylated.
TargetIl4
PurificationIL-4 specific antibody was purified by affinity chromatography and then biotinylated
ConjugationBiotin

Storage & Handling

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StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLyophilized
Concentrationbatch dependent
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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BCGF, BCDF, B cell stimulating factor (BSF-1)

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Quality Guarantee

Quality Guarantee

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UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqP07750

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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0.025 mg
$ 750.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 5-10 working days
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