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Mouse Corticosterone (Cort) ELISA Kit

SKU: orb1146853
Free Kit Promotion

Description

The Mouse Corticosterone (Cort) ELISA Kit is designed to quantify Corticosterone (Cort) in mouse serum, plasma, urine and other biological fluids. The kit has a detection range of 1.57-100 ng/mL and a sensitivity of 0.5 ng/mL.

Research Area

Infectious Disease & Virology, Metabolism Research

Images & Validation

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Tested ApplicationsELISA
Application Notes
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse Cort. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Cort. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Cort in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

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ReactivityMouse
Sample Typesserum, plasma, urine and other biological fluids
Assay TypeCompetitive
Assay Time2h
Range1.57-100 ng/mL
Sensitivity0.5 ng/mL
Concentration100 ng/mL

Storage & Handling

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StorageRefer to the Storage Guidelines in the Manual
Expiration DatePlease enquire.
DisclaimerFor research use only

Alternative Names

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Corticosrone

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Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

Select a size below

48 T
$ 350.00
96 T
$ 490.00
DispatchUsually dispatched within 5-10 working days
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