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Mouse Tyrosine Kinase B (TRKB) ELISA Kit

SKU: orb1736470
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Description

The Mouse Tyrosine Kinase B (TRKB) ELISA Kit uses a sandwich enzyme immunoassay to quantify Tyrosine Kinase B (TRKB) in mouse tissue homogenates, cell lysates and other biological fluids. The kit has a detection range of 93.75-6000 pg/mL and a sensitivity of 38.83 pg/mL.

Research Area

Pharmacology & Drug Discovery

Images & Validation

Application Notes
standard: 6000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TRKB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TRKB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TRKB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TRKB in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

ReactivityMouse
Sample TypesTissue homogenates, cell lysates and other biological fluids.
Assay TypeSandwich
Assay Time3.5h
Range93.75-6000 pg/mL
Sensitivity38.83 pg/mL
Concentration6000 pg/mL

Storage & Handling

StorageRefer to the Storage Guidelines in the Manual
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Tyrosine Kinase B

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Protocol Information

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48 T
$ 330.00
96 T
$ 470.00
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