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Rabbit Anti-Goat IgG (H&L) Antibody (HRP)

SKU: orb699946

Description

Rabbit anti-Goat IgG (H&L), HRP conjugated - trial sample

Research Area

Cell Biology

Images & Validation

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Tested ApplicationsELISA, IHC, WB
Dilution Range1 : 10 000 -1 : 50 000 (ELISA), 1 : 500-1 : 5000 (IHC), 1 : 10 000 - 50 000 (WB)
ReactivityGoat
Application Notes
For blocking BSA and non-fat milk is recommended to be replaced by other blocking reagents, like donkey serum or commercial formulations which are free from bovine IgG.

Key Properties

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HostRabbit
ClonalityPolyclonal
ImmunogenPurified goat IgG, whole molecule
PurificationImmunogen affinity purified rabbit IgG.
ConjugationHRP

Storage & Handling

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StorageStore lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Form/AppearanceLyophilized
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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10 μl
$ 150.00
1 mg
$ 530.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 5-10 working days
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