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Rat Brain Natriuretic Peptide (BNP) ELISA Kit

SKU: orb1806845

Description

Rat Brain Natriuretic Peptide (BNP) ELISA Kit

Research Area

Neuroscience; Cardiovascular Research

Images & Validation

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Application Notes
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat BNP. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to Rat BNP. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat BNP in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

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ReactivityRat
Sample Typesserum, plasma, Tissue homogenate and Other biological samples
Assay TypeCompetitive
Assay Time1.5H
Range31.25-2000pg/mL
Sensitivity18.75 pg/mL

Storage & Handling

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StorageRefer to the Storage Guidelines in the Manual
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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NPPB, GC-B, B-Type Natriuretic Peptide, Natriuretic Peptide Precursor B

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Protocol Information

Available Sizes

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48 T
$ 310.00
96 T
$ 430.00
DispatchUsually dispatched within 5-10 working days
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