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Rat Thyroxine (T4) EasyStep ELISA Kit

SKU: orb1950004
Free Kit Promotion

Description

The Rat Thyroxine (T4) EasyStep ELISA Kit is designed to quantify Thyroxine (T4) EasyStep in rat serum, plasma. The kit has a detection range of 9.38-600 ng/mL and a sensitivity of 7 ng/mL.

Research Area

Metabolism Research

Images & Validation

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Tested ApplicationsELISA
Application Notes
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat T4 antigen, and the Rat T4 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat T4 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat T4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

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ReactivityRat
Sample TypesSerum, plasma
Assay TypeCompetitive
Assay Time1.5h
Range9.38-600 ng/mL
Sensitivity7 ng/mL
Concentration600 ng/mL

Storage & Handling

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StorageRefer to the Storage Guidelines in the Manual
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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3,5,3',5'-Tetraiodothyronine; L-Thyroxine; Levothyroxine
Quality Guarantee

Quality Guarantee

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Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

Select a size below

48 T
$ 350.00
96 T
$ 490.00
DispatchUsually dispatched within 5-10 working days
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