Simian Ab1-42(Amyloid Beta Peptide 1-42) ELISA Kit

SKU: orb3127044

Description

The Simian Ab1-42(Amyloid Beta Peptide 1-42) ELISA Kit is designed to quantify Simian Ab1-42(Amyloid Beta Peptide 1-42) in primate serum, plasma and other biological fluids. The kit has a detection range of 15.63-1000 pg/mL and a sensitivity of 5.32 pg/mL.

Research Area

Neuroscience

Images & Validation

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Item 1 of 1

Application Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian Ab1-42. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian Ab1-42. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian Ab1-42, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian Ab1-42 in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

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Reactivity	Primate
Sample Types	serum, plasma and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	15.63-1000 pg/mL
Sensitivity	5.32 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Amyloid beta 42(ABeta 42); amyloid beta peptide 1-42; Aβ1-42,amyloid βpeptide 1-42

Quality Guarantee

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Simian Ab1-42(Amyloid Beta Peptide 1-42) ELISA Kit

Description

Research Area

Images & Validation

Key Properties

Storage & Handling

Alternative Names

Quality Guarantee

Protocol Information

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