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Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 128.4 KDa. Observed: 170&100-130&45-50 KDa, reducing conditions
Human
Glu25-Thr932
C-Fc
1 mg, 10 μg, 50 μg, 500 μgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 102.5 KDa. Observed: 130&80-95&40-50 KDa, reducing conditions
Human
Glu25-Thr932
C-6His
500 μg, 1 mg, 10 μg, 50 μgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified)
Predicted: 34.4 KDa. Observed: 25-40 KDa, reducing conditions
Human
Arg100-Leu233 & Ser34-Ile179
N-8His & N-Flag
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 46.3 KDa. Observed: 55-75 KDa, reducing conditions
Cynomolgus
Ser22-Arg201
C-Fc
500 μg, 1 mg, 10 μg, 50 μgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 23.1 KDa. Observed: 45-50 KDa, reducing conditions
Mouse
Phe23-Arg216
C-6His
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.
Predicted: 39.1 KDa. Observed: 45-50 KDa, reducing conditions
Human
Phe78-Pro366
N-6His
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 82.7 KDa. Observed: 110-125 KDa, reducing conditions
Human
Trp28-Ala526
C-Fc
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 14.7 KDa. Observed: 20-25 KDa, reducing conditions
Mouse
Ala37-Asp161
C-Flag
1 mg, 10 μg, 50 μg, 500 μgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 14.5 KDa. Observed: 20 KDa, reducing conditions
Human
Lys36-Asp161
C-Flag
50 μg, 500 μg, 1 mg, 10 μgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 14.7 KDa. Observed: 16-30 KDa, reducing conditions
Mouse
Glu21-Leu142
C-6His
10 μg, 50 μg, 500 μg, 1 mg
