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Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 53.1 KDa. Observed: 70-90 KDa, reducing conditions
Mouse
Glu24-Ser478
C-6His
10 μg, 1 mg, 50 μg, 500 μgUnconjugated
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.
Predicted: 87.5 KDa. Observed: 120 KDa, reducing conditions
Mouse
Glu100-Gly647
C-Fc
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 32.5&11.6 KDa. Observed: 42-58&12 KDa, reducing conditions
Mouse
Ser22-Val301&Ile21-Met119
C-6His
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 62.7 KDa. Observed: 90-110 KDa, reducing conditions
Mouse
Ser27-Ala337 (Val192Ala)
C-Fc
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.
Predicted: 47.9 KDa. Observed: 58-70 KDa, reducing conditions
Mouse
Gln23-Gly206
C-Fc
50 μg, 500 μg, 1 mg, 10 μgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 17.9 KDa. Observed: 17 KDa, reducing conditions
Human
Met53-Leu214
N-6His
1 mg, 10 μg, 50 μg, 500 μgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 98.4 KDa. Observed: 125-160 KDa, reducing conditions
Mouse
Asn24-Asn637
C-Fc
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 36.6 KDa. Observed: 55-70 KDa, reducing conditions
Mouse
Ser27-Ala337
C-6His
10 μg, 50 μg, 500 μg, 1 mgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 33.6 KDa. Observed: 35 KDa, reducing conditions
Mouse
Asn43-Met330
N-6His
10 μg, 50 μg, 1 mg, 500 μgUnconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 50.8 KDa. Observed: 72 KDa, reducing conditions
Mouse
Val38-Lys245
C-Fc
10 μg, 50 μg, 500 μg, 1 mg
