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Description
Research Area
Metabolism Research
Images & Validation
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Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat Nb2-11 lymphoma cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 x107 IU/mg. |
| Endotoxins | Less than 0.1 EU/ug of rHuGH as determined by LAL method. |
| MW | Approximately 22 kDa, a single non-glycosylated polypeptide chain containing 191 amino acids. |
| Purity | > 96 % by SDS-PAGE and HPLC analyses. |
| Protein Sequence | FPTIPLSRLF DNAMLRAHRL HQLAFDTYQE FEEAYIPKEQ KYSFLQNPQT SLCFSESIPT PSNREETQQK SNLELLRISL LLIQSWLEPV QFLRSVFANS LVYGASDSNV YDLLKDLEEG IQTLMGRLED GSPRTGQIFK QTYSKFDTNS HNDDALLKNY GLLYCFRKDM DKVETFLRIV QCRSVEGSCG F |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.0, containing 3 % Mannitol, 0.02 % Tween-20. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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