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Description
Research Area
Immunology & Inflammation
Images & Validation
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Item 1 of 1
| Application Notes |
|---|
Key Properties
−| Source | Mammalian cell |
|---|---|
| Biological Origin | Homo sapiens (Human) |
| Biological Activity | ①Measured by its binding ability in a functional ELISA. Immobilized Human IL4 at 2 μg/mL can bind Biotinylated Human IL4R. The EC50 is 7.926-9.836 ng/mL. ②Human IL4 captured on Protein A Chip can bind Recombinant Human IL4R with an affinity constant of 17.1 nM as detected by MetaSPR Assay. |
| Tag | C-terminal hFc1-MyC-tagged |
| Expression Region | 25-153aa |
| Protein Length | Full Length of Mature Protein |
| MW | 43.9 kDa |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Protein Sequence | HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS |
Storage & Handling
−| Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
|---|---|
| Form/Appearance | Lyophilized powder |
| Buffer/Preservatives | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Interleukin-4; IL-4; B-cell stimulatory factor 1; BSF-1; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra
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UniProt
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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