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Description
Research Area
Cell Biology
Images & Validation
−Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Activity | Fully biologically active when compared to standard. The biological activity is tested by in vivo assay using healthy wild type male mice (C57BL/6J). |
| Endotoxins | Less than 1 EU/ug of rHuNesfatin-1 as determined by LAL method. |
| MW | Approximately 9.6 kDa, a single non-glycosylated polypeptide chain containing 82 amino acids. |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Protein Sequence | VPIDIDKTKV QNIHPVESAK IEPPDTGLYY DEYLKQVIDV LETDKHFREK LQKADIEEIK SGRLSKELDL VSHHVRTKLD EL |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Nucleobindin 2
Similar Products
−Recombinant Human Nucleobindin-2 Protein [orb2994237]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 9.6 KDa. Observed: 10 KDa, reducing conditions
10 μg, 1 mg, 50 μg, 500 μgHuman Nesfatin-1/NUCB2/Nucleobindin-2 ELISA Kit [orb154594]
Human
31.2 pg/ml - 2,000 pg/ml
<10 pg/ml
96 T

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Quick Database Links
UniProt
UniProt Details
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide


