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Description
Research Area
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Origin | Mouse |
| Biological Activity | ELISA: Immobilized Anti-Human GUCY2C mAb at 2μg/ml (100 μl/well) can bind Recombinant Mouse GUCY2C (C-Fc) (Cat#orb2992792)*.*: Biotinylated by NHS-biotin prior to testing. The ED50 of Recombinant Mouse GUCY2C (C-Fc) (Cat#orb2992792) is 18.75 ng/ml. (Regularly tested) |
| Tag | C-Fc |
| Expression Region | Val20-Met433 |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
| Conjugation | Unconjugated |
| MW | Predicted: 74 KDa. Observed: 70-145 KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of PBS, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Mouse GUCY2C Protein, His Tag [orb1826796]
Unconjugated
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 47.9 kDa after removal of the signal peptide. The apparent molecular mass of mGUCY2C-His is approximately 55-130 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μgRecombinant Mouse GUCY2C/GCC Protein, N-His [orb2963719]
ELISA, SDS-PAGE, WB
>90% as determined by SDS-PAGE.
44.95 kDa
1 mg, 50 μg, 100 μgRecombinant Mouse GUCY2C/GCC Protein, N-His [orb2824266]
>90% as determined by SDS-PAGE.
44.95 kDa
20 μg, 50 μg, 100 μg

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