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Recombinant Porcine Interleukin-1 Receptor Antagonist Protein
SKU: orb1906098
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Description
Research Area
Immunology & Inflammation; Pharmacology & Drug Discovery
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Key Properties
−| Source | Escherichia coli |
|---|---|
| Expression System | Escherichia coli |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 x104 IU/mg in the presence of 75 pg/ml rPoIL-1α. |
| Endotoxins | Less than 0.1 EU/ug of rPoIL-1RA as determined by LAL method. |
| MW | Approximately 17.1 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids. |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Protein Sequence | HPLGKRPCRM QAFRIWDVNQ KTFYLRNNQL VAGYLQGPNT KLEEKIDVVP VEPHFVFLGI HGGKLCLSCV KSGDEMKLQL DAVNITDLRK NSEQDKRFTF IRSDSGPTTS FESAACPGWF LCTALEADQP VGLTNTPKAA VKVTKFYFQQ DQ |
Storage & Handling
−| Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered concentrated solution in 1 x PBS, pH 7.4, 1 mM DTT. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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Protocol Information
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