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Description
Research Area
Cell Biology
Images & Validation
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Key Properties
−| Source | E. coli |
|---|---|
| Biological Activity | Assay 1: The ED50 was determined by the dose - dependent stimulation of IL - 8 production by Human PBMC is less than 10 ng/mL.Assay 2: TWEAK weakly induces the death of HT29 cells when cultured in the presence of IFN - γ. The ED50 for this effect is between 30 - 45 ng/mL. |
| Target | TNFSF12 |
| Purity | Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg). |
| Protein Sequence | MKGRKTRARR AIAAHYEVHP RPGQDGAQAG VDGTVSGWEE ARINSSSPLR YNRQIGEFIV TRAGLYYLYC QVHFDEGKAV YLKLDLLVDG VLALRCLEEF SATAASSLGP QLRLCQVSGL LALRPGSSLR IRTLPWAHLK AAPFLTYFGL FQVH |
Storage & Handling
−| Storage | The lyophilized TWEAK recombinant protein is stable for at least 2 years from date of receipt at -20°C. Reconstituted TWEAK is stable for at least 3 months when stored in working aliquots with a carrier protein at -20°C. As with any protein, exposing TWEAK recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
|---|---|
| Form/Appearance | Lyophilized |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−APO3L, DR3LG, TWEAK, APO3L, UNQ181/PRO207, Tumor necrosis factor ligand superfamily member 12, APO3 ligand
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Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_003800.1 |
|---|
Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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