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Description
Images & Validation
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Item 1 of 2
| Tested Applications | ELISA, WB |
|---|---|
| Dilution Range | ELISA: 1:10,000-1:50,000, WB: 1:1,000-1:5,000 |
| Reactivity | Camelus |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | This antibody was prepared from whole rabbit serum produced by repeated immunizations with a VHH camelid domain protein. |
| Purity | Anti-VHH is directed against the llama VHH protein fragment. The product was purified from monospecific antiserum by protein A chromatography. Anti-VHH antibody detects recombinant VHH proteins, native Llama IgG2 and native Llama IgG3. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.3 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-VHH Antibody, single-domain antibody (sdAb), camelid VHH antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)