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Description
Images & Validation
−| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | WB: 1:500~1:1000; IHC: 1:50~1:100; IF: 1:100~1:200 |
| Reactivity | Human, Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Peptide-KLH. Peptide sequence around aa.30~34/34~38 (H-D-S-G-L- D-S -M-K) derived from Human IkB-a |
| Target | IkB-a |
| Purification | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Concentration | 1.0mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−I-kappa-B-alpha; IKBA; NF-kappaB inhibitor alpha; NFKBI; NFKBIA
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Quick Database Links
Gene Symbol
IkB-a
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
Yang, Xifeng et al. Downregulation of miR‑181b inhibits human colon cancer cell proliferation by targeting CYLD and inhibiting the NF‑κB signaling pathway Int J Mol Med, 46, 1755-1764 (2020)











